Development of an open access, virtual bioinformatics lab for students in medical laboratory sciences.

OBJECTIVES: Despite the growing importance of bioinformatics in molecular diagnostics, not all medical laboratory sciences (MLS) programs provide instruction in this field. We developed and assessed a virtual laboratory learning unit to introduce basic bioinformatics concepts and tools to MLS students. METHODS: The unit included a video tutorial, written instructions for the online laboratory activity, and a postactivity review video. The effectiveness of the instruction was evaluated using preassessment and postassessment questions, performance of the online tasks, and a survey assessing the students' attitudes toward the learning unit. RESULTS: A prototype of the module was tested with 32 graduate and undergraduate students. Modifications were made based on the pilot test results and student feedback, and the refined version was subsequently evaluated with a different group of 20 undergraduate students. The participants responded favorably to the learning unit and successfully achieved the learning objectives, gaining familiarity with fundamental bioinformatics concepts and terminology, effectively employing basic computational tools, and developing an appreciation for the field. CONCLUSIONS: Our learning unit is a promising tool for introducing MLS students to the field of bioinformatics. As an open educational resource, it has the potential to be integrated into molecular biology education for MLS programs anywhere.

Effect of science virtual laboratory combination with demonstration methods on lower-secondary school students' scientific literacy ability in a science course.

Virtual laboratory is computer software that has the ability to perform mathematical modeling of computer equipment presented in the form of simulations. Virtual laboratory is not a substitute for real laboratory, but are used to complement and improve the weaknesses of real laboratory. This study aims to determine the effect of virtual laboratory combination with demonstration methods on lower-secondary school students' scientific literacy ability in a science course. The design of this research is quasi-experimental. The sample in this study was 102 students (12-14 years old) in a lower-secondary school in the city of Yogyakarta, Indonesia, used as experiment 1 group 1 (n = 34), experiment 2 group (n = 34), and control group (n = 34). The three groups (experiment 1, experiment 2, and control) were tested with pretest and posttest. Experiment 1 group used virtual laboratory combination with demonstration methods, experiment 2 group used only virtual laboratory, and the control group used only a demonstration method. Scientific literacy ability was measured using multiple-choice tests before and after treatment. Statistical tests on mixed methods ANOVA were used to determine how effective the use of virtual laboratory combination with demonstration methods was in improving scientific literacy ability. The research result based tests of Within-Subjects Effects showed that there is a difference between the pretest-posttest scores of scientific literacy ability (F = 10.50; p < 0.05) in each group. The results based pairwaise comparison show that the significance value is <0.05, and there is a significant increase in the pretest-posttest scores of scientific literacy ability in every group. The result of effect size (partial eta squared) shows that the experiment 1 group to increase scientific literacy ability is 84.5%; experiment 2 group is 78.5%; control group is 74.3%. So, it can be concluded that experiment 1 group (virtual laboratory combination with demonstration methods) provides the most effective contribution to improving scientific literacy ability when compared to experiment 2 group virtual laboratory only) and control group (demonstration methods only).

Developing a virtual laboratory module for forensic science degree programmes.

Laboratory work is essential in forensic science degree courses. They provide students with an opportunity to put theory into practice, as well as develop relevant professional laboratory skills through a case-based learning framework. Traditional laboratory instruction involves the use of a written laboratory script or manual that details the laboratory procedures and techniques for an experiment. Occasionally, instructors may provide a brief in-person demonstration of a critical aspect of the experimental procedure during the session. Since the coronavirus pandemic, the use of virtual laboratory (vLab) resources, such as video demonstrations, in teaching science practical skills has increased. These resources may be used alone or in combination with in-person laboratory sessions in a flipped learning model. Previous research has shown that vLab resources could enhance students' knowledge, confidence, and experience inside the laboratory. This study aimed to explore the perceptions and attitudes of forensic science students toward the use of a vLab module. Three videos were created in which procedures for carrying out presumptive tests, screening exhibits, and recording examinations were demonstrated. Seven undergraduate students enrolled at different stages of a forensic science degree programme were introduced to the vLab module and interviewed using a semi-structured interview approach. Through a thematic synthesis of the interview transcripts, we found that the implementation of an inclusive vLab module could enhance students' knowledge, confidence, and independence in carrying out forensic science laboratory procedures.

Pre-lab video demonstrations to enhance students' laboratory experience in a first-year chemical engineering class.

The limited capabilities of teaching laboratories, combined with an increasing number of students enrolled in university, require constant augmentation of instructional approaches. By enhancing laboratory demonstrations with digital technology, these structural issues can be addressed while at the same time enhancing student understanding and learning. Our case study focuses on the fermentation lab part of the Reaction Equilibria and Thermodynamics (RET) module, a first-year chemical engineering course at the University of Birmingham. Video demonstrations were used to introduce students to the laboratory set-ups and walk them through each step and technique. The video demonstrations allowed the students to attend the in-person lab sessions having established knowledge and understanding of the processes involved and the outcomes desired, which decreased the burden on the facilities and the staff. A knowledge-based quiz and a student survey conducted at the end of the module showed that the pre-lab videos encouraged more active participation in the laboratory sessions and reinforced learning. Approximately 70% of the students polled in the first survey conducted within this project felt more confident going into the laboratory sessions after watching the pre-lab videos and attempting the knowledge quiz, while 92% of the students polled in the second survey judged the pre-lab video sessions as beneficial to them. Overall, the teaching method has the potential to improve student participation and access, boost confidence and learning, and provided a more structured and flexible approach to laboratory learning outcomes.

Effectiveness of virtual laboratory vs. paper-based experiences to the hands-on chemistry practical in Tanzanian secondary schools.

Science subjects at pre-tertiary and tertiary education levels are important for socio-economic and industrial development of any country; however, they are difficult for students to construct their concepts. In Tanzania, insufficient or lack of practical experiments are major challenges for science subjects due to insufficient or lack of laboratories, apparatus, expertise or reagents. Thus, this research assesses the effectiveness of paper-based against virtual laboratory experiences towards improvement of real (hands-on) chemistry practical in Tanzanian secondary schools focusing in Dodoma region. Chemistry virtual laboratory was developed and being deployed at Dodoma Secondary School for students who were never done practical sessions before to avoid biasness towards the study. The students were divided into three groups, namely paper-based and real laboratory as control groups (CG) and virtual laboratory as the experiment group (EG). Each group was further divided into two groups for the rest approaches forming six (6) groups. For EG, students were taught based on instructional approach which was enriched by computer animations in the computer laboratory. Results indicate that students who firstly attended virtual laboratory performed better in real laboratory than those who firstly attended real laboratory. Furthermore, the best progressive learning and performance for real experiments appears when the virtual laboratory preceded paper-based practical experiments. Thus, virtual laboratory is a very useful tool for learning chemistry practical not only to schools without laboratories but also to those with laboratories; and it should be considered by all the pre-tertiary schools in Tanzania and other schools in similar situations.

A Comparison of Real and Virtual Laboratories for Pharmacy Teaching.

New approaches to teaching and learning in the tertiary setting offer students flexibility for learning and, in a pandemic, suggests ways to provide learning when face-to-face delivery cannot be conducted. Courses that contain a hands-on laboratory component can be resource intensive in terms of equipment, staff, and facilities, thus more difficult to deliver when hands-on laboratory work is precluded. This study developed two virtual laboratories that could be completed online and, using a crossover design, evaluated student learning outcomes from virtual and real laboratory activities for 57 students. It also gained student feedback on their learning experiences. Overall, student knowledge increased significantly for each topic after completing either the virtual or real laboratory activities. However, no significant difference in learning was observed when outcomes from virtual or real laboratories were compared. Feedback from students indicated that most students found online modules easier to follow, they provided better background information, and would be revisited, but real laboratories were more interesting. Reinforcing learning, understanding, and remembering processes were reportedly similar for both, indicating no negative impact when a virtual laboratory was used. This study provides supporting evidence for the use of virtual laboratories where the focus is on learning concepts and not on student proficiency at operating laboratory equipment.

Learning to SuRRF: Sustained Engagement and Increased Academic Productivity Over Two Years of a Resident-Led Virtual Laboratory.

OBJECTIVE: To determine if implementation of a resident-led virtual laboratory can sustain increased engagement and academic productivity in residents and faculty. DESIGN: We developed and introduced a multimodal virtual Surgery Resident Research Forum (SuRRF) in July 2019. SuRRF utilizes monthly virtual lab meetings, weekly newsletters, a centralized database of projects, project tracking tools, and a shared calendar of deadlines to facilitate research among surgical residents. Data on number of participating residents, faculty, and projects across SuRRF meetings at 1-year (7/2020) and 2-years post-implementation (9/2021) were collected to evaluate engagement. Institutional ACGME Resident Scholarly Activity and Faculty Scholarly Activity reports were evaluated for the pre-SuRRF implementation (2018-2019) and post-implementation (2020-2021) academic years to assess productivity pre- and post-implementation. SETTING: Three tertiary academic hospitals of a single health system in New York. PARTICIPANTS: All residents in our general surgery program during the study period, including research residents, were eligible to participate in our study. RESULTS: At 1-year, there were 2 attendings, 13 residents, and 23 projects, compared to 12 attendings, 25 residents, and 42 projects at 2-years post-implementation. Post-SuRRF implementation, residents had significantly more publications (0.56 ± 0.15 vs. 1.10 ± 0.15, p = 0.005), textbook chapters (0.00 vs. 0.010 ± 0.044, p = 0.014), research participation (p < 0.01), and scholarly activity (p = 0.02). Post-SuRRF, faculty had significantly more publications (0.74 ± 0.15 vs. 2.20 ± 0.33, p < 0.001) and scholarly activity (p = 0.006). CONCLUSIONS: SuRRF promotes exposure to projects and resources and increases collaboration and peer-to-peer mentorship. Our experience with SuRRF suggests that resident-led virtual laboratories may increase peer-reviewed publications and improve resident and faculty engagement in scholarly activity, thus supporting academic growth.

Teaching the "acid-base" subject in biochemistry via virtual laboratory during the COVID-19 pandemic.

Virtual laboratories have started to be the leading alternative teaching tools during the Covid-19 pandemic process. The "Acid-Base" subject is among those that form the foundations of biochemistry. Students can learn the "Acid-Base" subject in a secure environment, with remote access through the use of virtual laboratory simulations. Simulation was applied to fourth-year undergraduate students in Turkey who want to obtain a bachelor's degree in science teaching during the present study. During the application process, the students conducted different experiments on the concepts of strong acid, strong base and pH, which are the basic concepts of biochemistry. In addition, student opinions regarding the application were also obtained. It was thus determined that the majority of the students were able to write hypotheses, test their accuracy and report the test findings correctly; a small number of students started to apply the simulations after examining them in accordance with scientific terminology. At the end of the application, the students also stated that they had fun during the application which also provided effective learning. It is recommended that classroom activity which is integrated with simulation be used in Chemistry, Biochemistry and Science Laboratory Applications courses.

Interaction between cognitive styles and genders when using virtual laboratories and its influence on students of health college's laboratory skills and cognitive load during the Corona pandemic.

This study examined the interaction between cognitive style-gender within Virtual Laboratories (VL) and its influence on students of health college's Laboratory Skills (LS) and Cognitive Load (CL) during the Corona pandemic. This research method is a combination of quasi-experimental research and survey research; consisting of two male and two female experimental groups (contemplative and impulsive). Each group had 20 students from General Health colleges. In the third level, with the microbiological course, eight experiments were studied by a Virtual laboratory (Praxilabs) during the eLearning study in 2020's first semester. Results showed that VL-using Students of General Health colleges studying microbiology had better CL and LS, besides significantly distinguishing between males and females using VLs in CL and LS where males benefited more. Also, a significant difference was established between CS (contemplative/impulsive) VL-using students in CL and LS to the benefit of the contemplative cognitive style. There LS significantly differ due to gender-CS interaction; however, CL does not have any differences because of this interaction.

The use of virtual laboratory in teaching respiration subject in the Covid-19 process.

Along with the Covid-19 pandemic, universities in Turkey as well as all over the world have started to carry out their education activities with the distance education method. In this case, many difficulties were encountered in the application of the Biology course, which is one of the applied courses carried out in the laboratory. Virtual laboratory applications can be preferred especially in teaching abstract subjects that are difficult to understand such as respiration. The teacher can write the process steps and theory related to the experiment to be designed, and the students can follow these process steps and perform the experiment in an interactive way. In this way, students can have a real-like laboratory experience by following the experimental procedure with laboratory materials step by step.

Benefits and challenges in the implementation of virtual laboratory simulations (vLABs) for medical biochemistry in Indonesia.

The pandemic caused major shifts in the delivery of education worldwide. In the teaching of medical biochemistry, the greatest impact was towards the delivery of traditional laboratory simulations. In this study, we highlight the benefits and barriers encountered in the use of virtual laboratories (vLABs) to substitute traditional laboratory practicals. The subjects were a class of 271 medical students at the Faculty of Medicine, Hasanuddin University, all freshman undergoing the Biomedicine Block. The study assessed the use of a commercial vLAB on antibodies and blood typing procedures, which were implemented using our four-step model of vLAB implementation. Collected data include the lecturer-assigned pre- and post-test result, built-in vLAB assessment result of the student first and best attempts, a student perception questionnaire based on a 5-point Likert scale, and an open ended questionnaire regarding student perceptions of the advantages and disadvantages of the vLAB. We observed a remarkable increase of lecturer assigned pre- and post-test scores and built-in first and best attempt scores (p < 0.0001, Wilcoxon signed rank test). A majority of students reported increased motivation when using the vLABs, and favored the ability of mastery through repetition. However, technical and language barriers were highlighted by students during the vLAB implementation. We demonstrate a successful implementation of commercial vLABs in a cohort of non-native English speakers using our four-step approach. Implementation requires strong support from faculty to address technical and language barriers that arise during use of vLABs.

Virtual/Remote Labs for Fluorescent Immunocytochemistry or Western Blotting: The Next Best Thing to Being There.

The SARS CoV-2 pandemic forced many college courses to convert to remote instruction almost overnight in the middle of the spring 2020 teaching semester. This article presents two molecular biology labs formerly performed in person by students but converted into virtual labs. The virtual immunocytochemistry experiment teaches the specificity of antibody staining, principles of fluorescent microscopy, diversity of brain cell types and morphologies, and journal article Figure construction skills. The virtual Western blotting experiment teaches sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the specificity of antibody binding, and graph creation and interpretation skills. Both virtual experiments use professionally-produced web-based videos of scientists conducting the lab procedures. Students must answer questions about the techniques and analyze real experimental data generated by past students to take a quiz and write a journal article-style lab report. At the whole-class level, student quiz and lab report scores from these virtual labs were not statistically different from those from the in-person versions of the same labs from a previous semester, using t tests with the Bonferroni correction. On the virtual Western blot quiz, students who did the virtual version actually scored higher than students who did the in-person version. These results were significant when the 2020 data were analyzed by within-student paired t tests for in-person labs done before COVID-19 versus those done virtually after dismissal for all-remote instruction. The students learned the laboratory concepts and data analysis skills just as well virtually as their predecessors had in person. However, the students trained virtually reported that they could not enter the lab and actually do Western blotting or fluorescent immunocytochemistry with their own hands without extensive additional training. These virtual experiments can be done with data included in the supplemental materials or can easily be adapted for any micrographs or Western blotting images available from previous lab experiments, or in the published literature. When COVID-19 or other public health emergencies necessitate remote instruction and we can't use the best practice of hands-on lab work, virtual labs can be the next best thing to being there.

An Analysis of Health Science Students' Preparedness and Perception of Interactive Virtual Laboratory Simulation.

The achievement of learning goals via laboratory practical depends on both extrinsic and intrinsic factors. They could be limited by laboratory time, incurred cost, safety, self-efficacy, inadequate prior preparation by learners, and different learning styles. Hence, virtual laboratory simulation (vLAB) may be an appropriate e-learning tool to overcome these restrictions. In this study, student's perception of the usefulness of vLAB was determined by using deoxyribonucleic acid (DNA) gel electrophoresis and polymerase chain reaction (PCR) as case examples. The perception of Year 2 and 3 health science undergraduate students' (N = 87) was studied using a questionnaire consisting of 12 items, rated on a 5-point Likert-scale. The attainment of learning outcomes was assessed using pre-and post-tests containing multiple-choice questions (MCQs). In addition, student's experience and learning from the vLAB were further explored using qualitative analysis. Although there was no significant difference between the mean scores of the pre-and post-tests, results showed that all participants perceived vLAB well, with a median score of 4 (Agree) for all items in the questionnaire. It provides a meaningful learning experience and an authentic environment where students feel safe to practice what they have learnt in lectures. Moreover, vLAB facilitates individualised learning and enhances self-efficacy among students. In conclusion, vLAB prepares students for physical laboratory sessions by activating the prehension dimension of Kolb's learning cycle, therefore complementing and strengthening the attainments of health sciences laboratory learning goals and outcomes.

Diving Under the SuRRFace: Achieving More Than Just Compliance with ACGME Scholarly Activity Mandate.

OBJECTIVE: To determine if building a digital technology supported infrastructure improves general surgery residents' confidence to conduct clinical research. DESIGN: We developed and introduced a multimodal virtual Surgery Resident Research Forum (SuRRF) in July 2019. An anonymized survey asked residents to rate their confidence using a five-point Likert scale in various fields pre- and post-intervention. Fields included: finding a research mentor, developing a project, conducting research, performing an effective literature search, navigating internal and external resources, and ability to complete a research project. SETTING: Northwell Health - North Shore University Hospital / Long Island Jewish Medical Center: academic tertiary care centers. PARTICIPANTS: All 58 residents in our general surgery program, including research residents, were eligible to participate in our study. RESULTS: Survey response rate was 55% (28 clinical residents, 4 research fellows). Post-implementation of SuRRF, all respondents (PGY1-5) reported an increase in awareness of abstract/conferences submission deadlines (2.34 ± 1.1 pre- vs. 3.75 ± 1.1 post-implementation, p = 0.004) and ability to navigate institutional electronic medical information library resources (2.2 ± 1.0 pre- vs. 3.62 ± 1.2 post-implementation, p = 0.000). Junior residents (PGY1-3) had improvement in all areas except for finding a mentor and improving their confidence with literature review. CONCLUSIONS: Creation of a resident-led virtual laboratory infrastructure increases participation, improves perception of research abilities, and improves attitudes towards performing clinical research among general surgery residents. Future research will follow the impact of this virtual laboratory on publications and grants.

Exploring the use of virtual laboratory simulations before, during, and post COVID-19 recovery phase: An Animal Biotechnology case study.

This study presents an evaluation of integrating virtual laboratory simulations in assessment design of a biotechnology course at Taylor's University in Malaysia before, during and post-COVID recovery phases. The purpose was to investigate how virtual laboratory simulations were integrated as part of the assessments of a practical-embedded course-the aim being to evaluate students' acceptance and perception of using virtual simulation. A total of 46 students, across three different study cohorts (August 2019, March 2020, and August 2020) were evaluated different educational aspects of using virtual laboratory cases in a 4-week course within Animal Biotechnology. Overall, students regarded virtual laboratory simulation useful as part of their learning, and there is a significant increase in the level of acceptance before, during and post-COVID recovery phases. The study showed that across the different study cohorts, students perceived their confidence level in laboratory skills have been enhanced and that they can apply the skills in real-life situation. Interestingly, students (March and August 2020 cohort) who have not been exposed to the related laboratory session still perceived that the simulated activity provides clear explanation and realistic experience. Furthermore, it had been highlighted across the study cohorts that the quiz questions helped to enhance their understanding on the underlying principles of the laboratory techniques. The overall conclusion of this study was that structured simulation-based activities which provide clear instructions and explanation would support significant improvements in students learning.

Are virtual physiology laboratories effective for student learning? A systematic review.

It is unclear if the transition from traditional, in-person physiology laboratories to virtual alternatives has educational impacts on students. This study used a systematic review to critically evaluate research papers that investigated the effectiveness of virtual physiology laboratories for student learning. Eleven studies, retrieved from the Education Resources Information Center (ERIC) and Ovid MEDLINE databases, were selected for inclusion in this review, based on predetermined eligibility criteria. Subsequently, the studies went through a power analysis for potential biases before their results were synthesized and analyzed. This systematic review found that virtual physiology laboratories are effective for students' learning of concepts. However, it was inconclusive as to whether virtual physiology laboratories are effective for students' motivation for learning and learning of technical skills. It was found that blended models of virtual laboratories are at least as effective as in-person laboratories for conceptual learning. Overall, this systematic review provides useful insights for educators regarding the educational impacts of implementing virtual laboratories into the physiology curriculum and suggests research models for future evaluation of virtual laboratories.

Virtual protein quantification laboratory enhancing online teaching.

Virtual laboratory simulations can contribute to the educational objectives related to practical classes, especially in situations of online or hybrid teaching. We present a proposal for laboratory activity involving the quantification of proteins with the biuret reaction and measurement by visible light spectrometry. The student will be able to develop the procedures and obtain the absorbance values for calculations and discussions using the ProtVirt software, integrating its use with the teacher's pedagogical proposal.

Design of an affordable IoT open-source robot arm for online teaching of robotics courses during the pandemic contingency.

This article explains the design and construction of an affordable, open-source robot arm for online teaching of robotics courses. The main goal of the proposed robotic prototype is to deal with the current situation of pandemic contingency, where students and instructors cannot attend laboratory facilities in person. The robotic system has four main components: an electromechanical robot arm structure, a control system, a Wi-Fi communications module, and a human-machine interface. The IoT (Internet of Things) robot arm can be used to demonstrate important robotics topics such as direct and inverse kinematics, which are shown by programming simple and complex motions using the Denavit-Hartenberg (DH) methodology. The capabilities of the robotic system are empowered by IoT technology, which is demonstrated with an HMI interface deployed in a smartphone using wireless Wi-Fi communication through an ESP32 microcontroller. The arm's purpose is to be a low-cost and replicable robot that aids the comprehension of robotics design through project-based learning, from the theoretical aspects to the actual coding and construction of a prototype.

Trends in research on the organization of an inclusive education environment

The access to education for children and young people with special needs is an indicator of the humanization of society, a sign that this society can care for one of its most vulnerable groups. This paper reviews the principles and trends in studying the issues of organising an inclusive education environment. Particular emphasis is placed on the scientific novelty of the research, research methods and goals, the main purpose of which is to create a comfortable educational environment and find a solution to the problems of inclusive space. Further, the authors provide a detailed analysis of the project content in the conditions of Kazakhstan. They develop an experimental model of a spatial solution for an inclusive education environment. The results of this study contribute to the architectural and compositional improvement of educational buildings, increasing their comfort. The paper analyzes the role of the virtual environment in the development of experimental projects. It contains a diagram of the project time frame, a sample schedule and a description of the research group composition.

A virtual experiment improved students' understanding of physiological experimental processes ahead of a live inquiry-based practical class.

Physiology is commonly taught through direct experience and observation of scientific phenomena in "hands-on" practical laboratory classes. The value of such classes is limited by students' lack of understanding of the underlying theoretical concepts and their lack of confidence with the experimental techniques. In our experience, students follow experimental steps as if following a recipe, without giving thought to the underlying theory and the relationship between the experimental procedure and the research hypotheses. To address this issue, and to enhance student learning, we developed an online virtual experiment for students to complete before an inquiry-based practical. The virtual experiment and "live" practical laboratory were an investigation of how autonomic nerves control contractions in the isolated rabbit ileum. We hypothesized that the virtual experiment would support students' understanding of the physiological concepts, as well as the experimental design associated with the practical. Anonymous survey data and usage analytics showed that most students engaged with the virtual experiment. Students thought that it helped them to understand the practical physiological concepts and experimental design, with self-reported time spent on the virtual experiment (and not on lectures or practical class notes) a significant predictor of their understanding. This novel finding provides evidence that virtual experiments can contribute to students' research skills development. Our results indicate that self-paced online virtual experiments are an effective way to enhance student understanding of physiological concepts and experimental processes, allowing for a more realistic experience of the scientific method and a more effective use of time in practical classes.